Research progress in Guanxin Tongluo capsule for the treatment of coronary heart disease / 中国基层医药

Guanxin Tongluo capsule is a compound traditional Chinese medicine preparation for the treatment of coronary heart disease. The underlying mechanism may be related to expanding coronary artery, increasing coronary artery blood flow, reducing myocardial oxygen consumption, and decreasing whole blood viscosity, plasma viscosity, and hematocrit. This paper reviews the clinical research progress of Guanxin Tongluo capsule in the treatment of coronary heart disease from the aspects of etiology, pathogenesis, pharmacological research, and clinical research, providing evidence for scientific research and clinical application of Guanxin Tongluo capsule.

Bactericidal and Smear Layer Removal Efficacy of Herbal Alternatives Against Enterococcus Faecalis Dentinal Biofilm - An ex-vivo Study

Abstract Objective: To assess the antibacterial and smear layer removal ability of Trigonella foenum, Syzygium cumini, Terminalia chebula seed extracts against E. faecalis dentinal biofilm. Material and Methods: Agar well diffusion, micro broth dilution assay and time-kill curve assay were performed to determine the antibacterial activity. The ability of the herbal extracts to remove the smear layer on the root canal surface was assessed by scanning electron microscopy. Results: Antibacterial activity was observed for the extracts of S. cumini and T. chebula on E. faecalis dentinal biofilm and its planktonic counterparts. The smear layer was efficiently removed by the seed extracts of T. chebula alone. Seed extracts of T. foenum neither possessed antibacterial effect nor smear layer removal ability. Conclusion: The extracts of T. chebula seeds may replace conventional irrigant due to its antibacterial properties and smear layer removing the ability. The extracts of S. cumini may be used as an intracanal medicament as it exhibited a bactericidal effect against the E. faecalis dentinal biofilm following 18 hours of incubation.

Uso de la manzanilla en el tratamiento de las enfermedades periodontales / Chamomile use in the treatment of periodontal diseases

RESUMEN Fundamento: existe una gama de productos naturales, dentro de los que están los fitoterapéuticos, donde se destaca la manzanilla para tratar la enfermedad periodontal inflamatoria debido a la acción antiinflamatoria, cicatrizante, antialérgica, analgésica, antiséptica y bacteriostática que esta planta posee. Se ha demostrado que la manzanilla actúa también sobre la placa dentobacteriana, principal factor de riesgo de la enfermedad periodontal y es utilizada para el tratamiento de procesos agudos y crónicos periodontales. Objetivo: establecer las bases científicas del empleo de la manzanilla en el tratamiento de las enfermedades periodontales. Métodos: la búsqueda de la información se realizó en un periodo de seis meses y se emplearon las siguientes palabras: manzanilla, matricaria recutita; a partir de la información obtenida se realizó una revisión bibliográfica de un total de 150 artículos publicados en las bases de datos PubMed, Hinari, SciELO y Medline mediante el gestor de búsqueda y administrador de referencias EndNote, de ellos se utilizaron 52 citas seleccionadas para realizar la revisión, 40 de ellas de los últimos cinco años. Resultados: la manzanilla es nativa en muchos países de Europa, y se cultivan en países como Alemania, Egipto, Francia, España, Italia, Marruecos, y en partes de Europa del Este. Las diversas plantas de manzanilla son muy distintas y requieren su propio conjunto de condiciones para crecer. Sus propiedades, acciones terapéuticas y principios activos varían de una especie a otra. Conclusiones: la Matricaria chamomilla y la Phania matricarioides son de probada eficacia terapéutica en el tratamiento de enfermedades periodontales agudas y crónicas.

ABSTRACT Background: there is a range of natural products, including phytotherapeutic, which highlights the chamomile to treat inflammatory periodontal disease due to the anti-inflammatory, healing, antiallergic, analgesic, antiseptic and bacteriostatic that this plant possesses. It has been shown that chamomile also acts on dentobacterial plaque, the main risk factor for periodontal disease and is used for the treatment of acute and chronic periodontal processes. Objective: to establish the scientific basis of the use of chamomile in the treatment of periodontal diseases. Methods: the search of the information was carried out in a period of six months and the following words were used: chamomile, matricaria recutita; Based on the information obtained, a bibliographic review was made of a total of 150 articles published in the PubMed, Hinari, SciELO and Medline databases by means of the search manager and the EndNote reference manager. Of these, 52 selected citations were used to perform the review, 40 of them from the last five years. Results: Chamomile is native to many countries in Europe, and is grown in countries such as Germany, Egypt, France, Spain, Italy, Morocco, and in parts of Eastern Europe. The various chamomile plants are very different and require their own set of conditions to grow. Its properties, therapeutic actions and active principles vary from one species to another. Conclusions: Matricaria chamomilla and Phania matricarioides are of proven therapeutic efficacy in the treatment of acute and chronic periodontal diseases.

Anti-Cancer and Anti-Proliferation Activity of Ethyl Asetat Extract From Ant Nest (Myrmecodia pendans) in Burkitt's Lymphoma Cancer Cells

Objective: To determine the activity of anti-cancer and anti-proliferation of ethyl acetate fraction of ant nest plants (Myrmecodia pendans) in Burkitt's Lymphoma cancer cells. Material and Methods: The study was conducted in a pure laboratory experimental method using Burkitt's Lymphoma cancer cell culture. Gradual research begins with the determination, extraction and fractionation of ant nest plants, to test for proliferation barriers. Data analysis using two-way ANOVA followed by Post Hoc LSD test with a significance level of 95%. Pearson correlation test was conducted. Results: The results of testing the inhibition of Burkitt's Lymphoma cell proliferation with ethyl acetate extract treatment showed that there was inhibition of cell growth based on the concentration given, starting from the lowest concentration of 15.625 µg/mL. Likewise, the incubation time factor of 24, 48, and 72 hours showed that the longer the incubation time, the greater the inhibition of cell growth. Antiproliferation analysis of flavonoid ethyl acetate extract based on concentration and incubation time on absorption of optical density Burkitt's Lymphoma was statistically significant (p = 0.00). Conclusion: Ant nest ethyl acetate extract has the effect of proliferation inhibition on Burkitt's lymphoma cells.

An Herbal Alternative to Control Nosocomial Pathogens in Aerosols and Splatter During Ultrasonic Scaling

Abstract Objective: To evaluate the efficacy of herbal mouthwash (Himalaya Hiora Regular) against methicillin-resistant Staphylococcus aureus and Acinetobacter baumanni during ultrasonic scaling. Material and Methods: Group B (n=25) received herbal mouthwash and Group A (n=25) received 0.12% chlorhexidine mouthwash respectively as a preprocedural rinse. The aerosols produced by the ultrasonic unit were collected on MeReSa and Leeds Acinetobacter Agar plates. The experimental setting included eight different locations covering all areas of the operatory. The plates exposed to aerosols for a period of 30 minutes were incubated aerobically at 37ºC for 48hrs and the colony forming units (CFU) were statistically analyzed Results: Herbal mouthwash (Himalaya Hiora Regular) showed a significant reduction in mean CFU of MRSA compared to 0.12% chlorhexidine. While herbal mouthwash was on par with 0.12% chlorhexidine in the reduction of A. baumannii Conclusion: Herbal mouthwash was found to be more effective against MRSA than 0.12% Chlorhexidine mouthwash as a pre-procedural rinse. Both herbal mouthwash and chlorhexidine mouthwash was found to be effective against A. baumannii. Herbal mouthwash may be a safe alternative to chlorhexidine against nosocomial pathogens like MRSA and A. baumannii.

Research progress in pollen preparation for the treatment of benign prostatic hyperplasia / 国际生物医学工程杂志

Benign prostatic hyperplasia (BPH) is one of the common urinary system diseases in middle-aged and elderly men, which seriously affects the health and quality of life of patients. At present, the pathogenesis of BPH is still unclear, which critically affects the effective treatment of BPH. In recent years, more and more animal experiments and clinical trials have shown that pollen preparations have a significant inhibitory effect on BPH. In this paper, the pathogenesis of BPH and the related researches on the treatment of BPH by pollen preparations were reviewed, and the effective components of pollen preparations were analyzed, so as to discover the mechanism of BPH inhibition by pollen preparations and finally achieve the purpose of comprehensive utilization.

Evaluación preclínica de la actividad hepatoprotectora de Ocimum basilicum L. y Allium sativum L / Preclinical Evaluation of Hepatoprotective Activity of Ocimum basilicum L. and Allium sativum L

Fundamento: la búsqueda de alternativas de tratamiento naturales, orientadas a proteger el hígado de los efectos nocivos de hepatotoxinas, es un tema importante dentro de las investigaciones médico-farmacéuticas. Objetivo: evaluar preclínicamente la actividad hepatoprotectora de las especies Ocimum basilicum L. y Allium sativum L. en un biomodelo de toxicidad inducido por paracetamol. Métodos: se realizó un estudio farmacológico preclínico para evaluar el efecto hepatoprotector de las especies Ocimum basilicum L. y Allium sativum L. frente a la toxicidad inducida por el paracetamol. Se emplearon ratones adultos machos NMRI a los que se administró por vía oral extractos blandos de las plantas de estudio a dosis de 200mg/kg y 400mg/kg, tres días consecutivos previos a la inducción de la hepatotoxicidad. Se evaluaron los signos clínicos de toxicidad, parámetros bioquímicos hepáticos; también se realizó el análisis morfológico del hígado. Resultados: los parámetros bioquímicos analizados mostraron diferencias altamente significativas, pero solo tres grupos presentaron un comportamiento similar al grupo control no tratado. No se confirmaron alteraciones macroscópicas del hígado. Microscópicamente, predominó la ausencia de alteraciones histopatológicas en los grupos donde se administró Ocimum basilicum L. a ambas dosis y Allium sativum L. a 200mg/Kg; también se observó daño leve al administrar 400mg/Kg de Allium sativum L, con diferencias significativas respecto al grupo control no tratado. Conclusión: los extractos blandos de las especies Ocimum basilicum L. y Allium sativum L. a 200mg/kg, poseen mayor actividad hepatoprotectora frente a la acción tóxica del paracetamol.

Background: finding natural treatments designed to protect the liver from the damaging effects of hepatotoxins is an important topic in medical and pharmaceutical research. Objective: to pre-clinically evaluate the hepatoprotective activity of the species Ocimum basilicum L. and Allium sativum L. in an animal model of acetaminophen-induced toxicity. Methods: a preclinical pharmacological study was conducted to evaluate the hepatoprotective effect of the species Ocimum basilicum L. and Allium sativum L. against acetaminophen-induced toxicity. Adult male NMRI mice were used. They were orally administered soft extracts of the plants at doses of 200mg/kg and 400mg/kg for three consecutive days before inducing hepatotoxicity. Clinical signs of toxicity and hepatic biochemical parameters were evaluated; in addition, the morphological analysis of the liver was performed. Results: the biochemical parameters showed highly significant differences, but only three groups had results similar to the untreated control group. No macroscopic changes in the liver were confirmed. Microscopically, the absence of histopathological changes prevailed in the groups treated with Ocimum basilicum L. at both doses and Allium sativum L. at 200mg/kg; slight damage was observed by administering 400mg/kg of Allium sativum L., with significant differences from the untreated control group. Conclusions: soft extracts of the species Ocimum basilicum L. and Allium sativum L. at a dose of 200mg/kg have greater hepatoprotective activity against the toxic action of acetaminophen.

Confirmación de dexametasona y diclofenaco por LC-MS-MS como adulterantes en un producto herbolario / Dexamethasone and diclofenac by LC-MS-MS as herbal product adulterants

Objetivo. Confirmar la presencia de dexametasona y diclofenaco como adulterantes de un producto comercializado como de origen natural. Material y métodos. Para la identificación y confirmación de la presencia de los fármacos se utilizó un método de análisis instrumental por cromatografía de líquidos de alta presión acoplado a espectrometría de masas en tándem. Resultados. En el análisis de 11 frascos de Reumofan Plus obtenidos de pacientes y médicos de la localidad se confirmó la presencia de dexametasona y diclofenaco. La metodología utilizada permitió separar los esteroisómeros dexametasona y betametasona, las abundancias relativas de iones productos 237.2 y 279.2 m/z permiten diferenciar espectralmente un compuesto de otro. Conclusiones. Se confirmó la presencia de dexametasona y diclofenaco en muestras de un producto comercializado como "100% natural" obtenidas de diferentes pacientes o médicos en el periodo enero a diciembre de 2011.

Objective. To confirm the presence of dexamethasone and diclofenac as adulterants of an herbal product. Materials and methods. For identificaction and confirmation of drugs a method of instrumental analysis by liquid chromatography coupled with high pressure tandem mass spectrometry was used. Results. The presence of dexamethasone and diclofenac was confirmed in samples of 11 bottles of Reumofan Plus obtained from patients and/or physicians. The methodology used, allowed separation of stereoisomers dexamethasone and betamethasone, the relative abundances of product ions 237.2 and 279.2 m / z spectrally differentiate the compounds. Conclusions. The presence of dexamethasone and diclofenac was confirmed in samples of a product marketed as "100% natural" obtained from patients and / or physicians in a period from January to December, 2011.

Análise da eficácia do óleo de hortelã pimenta (Mentha piperita L) na síndrome do intestino irritável: revisão baseada em evidência / Analysis of the efficacy of the peppermint oil (Mentha piperita L) on irritable bowel syndrome: an evidence-based review / Análisis de la eficacia del aceite de menta (Mentha piperita L) sobre el síndrome del intestino irritable: revisión basada en evidencia

Objetivo: Avaliar a eficácia do óleo de hortelã-pimenta (Mentha piperita L) no tratamento de indivíduos com síndrome do intestino irritável (SII), analisando a melhora dos sintomas e da qualidade de vida. Métodos: Foram realizadas pesquisas nas bases de dados Trip database, National Guideline Clearinghouse, Guidelines finder, Cochrane Library, Dare, Bandolier e Medline utilizando os termos MeSH Irritable bowel syndrome e peppermint oil. Foram incluídas normas de orientação clínica (NOC), revisões sistemáticas e metanálises e ensaios clínicos aleatorizados e controlados (ECAC) que avaliassem a eficácia do óleo de hortelã-pimenta (OHP) no alívio sintomático ou melhoria da qualidade de vida de indivíduos com SII, quando comparado com placebo ou outra terapêutica validada. Resultados e Discussão: Foram selecionados 8 artigos (5 NOC, 3 revisões sistemáticas/metanálises). A evidência sugere que o OHP é eficaz no alívio sintomático global da SII, especialmente quando existe dor abdominal, verificando-se a sua superioridade em relação ao placebo na maioria dos estudos avaliados ­ com odds ratio favorecendo o óleo de hortelã-pimenta atingindo 2,7 (IC de 95% de 1,6 a 4,8) e NNT de 3. Existe também evidência de melhoria da qualidade de vida com o OHP, em comparação com o placebo (p<0,001). Conclusão: A evidência demonstra eficácia do OHP na SII, especialmente quando existe um componente de dor abdominal, quando comparado com o placebo (força de recomendação B). São necessários mais estudos com qualidade metodológica que avaliem eficácia e segurança a longo prazo do OHP no alívio sintomático da SII.

Objective: To evaluate the efficacy of peppermint oil (Mentha piperita L.) on individuals diagnosed with irritable bowel syndrome (IBS) regarding symptom improvement and quality of life enhancement. Methods: Literature search was conducted according to evidence based on methodology review at Trip database, National Guideline Clearinghouse, Guidelines finder, Cochrane Library, Dare, Bandolier and Medline, as well as using the MeSH index terms "Irritable bowel syndrome" and "peppermint oil". We selected practice guidelines, systematic reviews, meta-analysis and randomized controlled trials that evaluated peppermint oil (PO) efficacy in improving symptoms and/or quality of life when compared to placebo or other approved therapy for IBS. Results and Discussion: A total of eight articles were selected including five practice guidelines and three systematic reviews/ meta-analysis. Existing evidence suggests that there are enough data to support the use of peppermint oil in IBS for overall symptomatic relief, especially when abdominal pain is the dominant symptom, when compared to placebo (with an odds ratio of 2.7; CI 95%, 1.6 to 4.8 and NNT of 3). There is also evidence regarding overall quality of life improvement when using PO compared to placebo (p<0.001). Conclusion: So far, evidence suggests that PO should be considered for IBS patients, especially in the presence of abdominal pain (Strength of recommendation B). However, more high methodological quality studies that evaluate long-term efficacy and security of PO are needed.

Objetivo: Evaluar la eficacia del aceite de menta (Mentha piperita L.) en el tratamiento de pacientes con síndrome del intestino irritable (SII) y analizar el alivio de sus síntomas y la mejora en su calidad de vida. Métodos: Se realizaron búsquedas en las bases de datos Trip database, National Guideline Clearinghouse, Guidelines finder, Cochrane Library, Dare, Bandolier y Medline usando los términos MeSH "Irritable bowel syndrome" y "peppermint oil". Se incluyeron las normas de orientación clínica (NOC), revisiones sistemáticas y meta-análisis y ensayos clínicos controlados aleatorizados (ECAC) para evaluar la eficacia del aceite de menta (AM) en el alivio sintomático y mejora de la calidad de vida de las personas con SII en comparación con el placebo u otra terapia validada. Resultados y Discusión: Se seleccionaron ocho estudios (cinco NOC y tres revisiones sistemáticas/meta-análisis). La evidencia sugiere que el AM es eficaz en el alivio de los síntomas generales del SII, en particular si había dolor abdominal, verificandose su superioridad sobre el placebo en la mayoría de los estudios evaluados (odds ratio de 2,7; IC 95% de 1,6 a 4,8 y NNT de 3). Hubo también evidencia de mejora en la calidad de vida en los pacientes tratados con AM, en comparación con el placebo (p <0,001). Conclusión: Las pruebas demuestran la eficacia del AM en el SII, especialmente en presencia de dolor abdominal, en comparación con el placebo (fuerza de recomendación B). Se necesitan más estudios con buena calidad metodológica que evalúen la eficacia y la seguridad a largo plazo del AM en el alivio de los síntomas del SII.

Atividade antibacteriana de tinturas à base de plantas sobre microrganismos do biofilme dental / Antibacterial activity of plant-based tinctures on dental biofilm's microrganisms

Objetivo: Avaliar a atividade antibacteriana das Tinturas (T) de Salvia officinalis - T1, Malva sylvestris - T2 e do Anacardium occidentale - T3 sobre Streptococcus mutans (ATCC 25175) e Streptococcus oralis (ATCC 10557). Material e Método: Determinou-se a Concentração Inibitória Mínima (CIM) das tinturas pela técnica de microdiluição. Utilizaram-se microplacas com 96 poços. Em cada poço, foram inseridos 100μL de caldo Brain Heart Infusion duplamente concentrado, 100μL das tinturas em concentrações que variaram de 100 a 0,78mg/mL e 10μL de inóculo bacteriano (108 UFC/mL). As microplacas foram incubadas a 37°C, por 24 horas. A CIM correspondeu à última diluição na qual se verificou ausência de precipitado bacteriano ou turvação do meio de cultura. Os testes foram realizados em triplicata, a Clorexidina 0,12% (1,2mg/mL) serviu de controle, e os dados foram analisados descritivamente. Resultados: Sobre S. mutans e S. oralis, a CIM para cada tintura foi, respectivamente, 3,12 e 0,78 (T1); 6,25 e 3,12 (T2); e 1,56 e 0,78 (T3). Clorexidina 0,12% apresentou atividade satisfatória frente às cepas testadas. Conclusão: As tinturas avaliadas apresentaram atividade antibacteriana sobre as cepas de Streptococcus spp ensaiadas.

Objective: The aim was to evaluate the Minimum Inhibitory Concentration (MIC) of tinctures from Salvia - T1 (Salvia officinalis), Malva - T2 (Malva sylvestris) and Purple Cashew - T3 (Anacardium occidentale) against Streptococcus mutans (ATCC 25175) and Streptococcus oralis (ATCC 10557). Material and Method: The MIC of tinctures was determined through the microdilution technique using 96-wells microplates. To each well were placed 100mL of BHI doubly concentrated, 100μL of the tinctures in concentrations that ranged from 100 to 0.78 mg/mL and 10 mL of microbial inoculum (1.5x108 UFC/mL). The microplates were incubated at 37°C for 24 hours. The MIC corresponded to the last dilution in which there was no presence of bacterial precipitate or turbidity in the culture medium. Chlorhexidine at 0,12% (1,2mg/mL) was used as control. Results: For S. mutans and S. oralis the MIC for each tincture were respectively 3.12 and 0.78 (T1), 6.25 and 3.12 (T2), 1.56 and 0.78 (T3). Chlorhexidine 0.12% presented satisfactory activity against the studied strains. Conclusion: The analyzed tinctures presented antibacterial activity against the evaluated strains of Streptococcus.

Effects of broccoli extract on biodistribution and labeling blood components with 99mTc-GH / Efeitos do extrato de brócolis na biodistribuição e marcação dos componentes do sangue com 99mTc-GH

PURPOSE: People consume vegetables without the knowledge of the side effects of the biological and chemical contents and interactions between radiopharmaceuticals and herbal extract. To this end, current study is focused on the effects of broccoli extract on biodistribution of radiolabeled glucoheptonate (99mTc-GH) and radiolabeling of blood components. METHODS: GH was labeled with 99mTc. Quality control studies were done utilizing TLC method. Biodistribution studies were performed on male rats which were treated via gavage with either broccoli extract or SF as control group for 15 days. Blood samples were withdrawn from rats' heart. Radiolabeling of blood constituents performed incubating with GH, SnCl2 and 99m Tc. RESULTS: Radiochemical yield of 99mTc-GH is 98.46±1.48 percent (n=8). Biodistribution studies have shown that according to the control, the treated group with broccoli has approximately 10 times less uptake in kidney. The percentage of the radioactivity ratios of the blood components is found to be same in both groups. CONCLUSIONS: Although there is no considerable effect on the radiolabeling of blood components, there is an outstanding change on the biodistribution studies especially on kidneys. The knowledge of this change on kidney uptake may contribute to reduce the risk of misdiagnosis and/or repetition of the examinations in Nuclear Medicine.

OBJETIVO: As pessoas consomem verduras sem o conhecimento dos efeitos colaterais dos conteúdos biológicos e químicos e interações entre os medicamentos radiofarmacêuticos e os extratos vegetais. Para este fim, o estudo atual é focado sobre os efeitos do extrato de brócolis na biodistribuição do fármaco glucoheptonato (99mTc-GH) e da marcação de componentes do sangue. MÉTODOS: GH foi marcado com 99mTc. Estudos de controle de qualidade foram feitos utilizando o método do TLC. Os estudos de biodistribuição foram realizados em ratos machos que foram tratados por gavagem com um extrato de brócolis ou SF como grupo controle para 15 dias. Amostras de sangue foram retiradas do coração de ratos. Marcação de constituintes sanguíneos realizados incubação com SnCl2 GH e 99mTc. RESULTADOS: Radioquímica rendimento de 99mTc-GH é 98,46 ± 1,48 por cento (n = 8). Os estudos de biodistribuição mostraram que de acordo com o controle, o grupo tratado com brócolis tem aproximadamente 10 vezes menor absorção no rim. O percentual do ratio de radioatividade dos componentes do sangue é encontrado para ser igual nos dois grupos. CONCLUSÕES: Embora não haja nenhum efeito considerável sobre a marcação dos componentes do sangue há uma mudança notável na biodistribuição especialmente nos rins. O conhecimento desta mudança na captação de rim pode contribuir para reduzir o risco de erro diagnóstico e/ou a repetição dos exames de Medicina Nuclear.

Efeitos do uso de manjericão (Ocimum basilicum L) no perfil bioquímico de ratos Wistar / Effects of the use of basil (Ocimum basilicum L) in biochemical profile of Wistar rats

Objetivo - O manjericão (Ocimum basilicum L.) é uma planta comumente utilizada na culinária e tem sido apontado pelas suas propriedades benéficas à saúde, possuindo importantes efeitos antioxidantes, anti-inflamatórios e hepato-protetores. O objetivo deste trabalho foi avaliar os efeitos da administração de manjericão (Ocimum basilicum L.) no perfil bioquímico de ratos. Método - Trinta ratos machos da linhagem Wistar foram utilizados. Os ratos foram divididos aleatoriamente em três grupos de 10 animais e receberam tratamentos ad libitum por um período de trinta dias. O primeiro grupo (G1) recebeu água ad libitum, o segundo e terceiro grupos (G2 e G3) receberam infusão de manjericão nas concentrações de 20g/L e 40g/L, respectivamente. Foram feitas análises de glicemia, colesterol total, HDL-c, triglicerídeos, proteína C reativa (PCR), creatinina, aspartato aminotransferase (AST) e alanina aminotransferase (ALT). Resultados - A utilização do Ocimum basilicum L. promoveu redução de 37,44% no índice de colesterol total e de 34,55% nos níveis de triglicerídeos, e aumento de 39% nos níveis de HDL-c em relação ao grupo controle. Considerando a glicemia houve redução de 11,40% e 19,36% utilizando concentrações de 20 g/L e 40 g/L do manjericão, respectivamente. Não houve alteração significativa nos níveis de creatinina, PCR e enzimas hepáticas. Conclusão - Os resultados sobre as variáveis bioquímicas são promissores, visto que as alterações podem beneficiar indivíduos em condições de hipercolesterolemia e hiperglicemia, auxiliando na prevenção e tratamento de distúrbios crônico-degenerativos. Sugere-se que novos estudos sejam realizados, como a análise de toxicidade da infusão de manjericão em ratos Wistar e determinação dos constituintes químicos da infusão.

Objective - Basil (Ocimum basilicum L.) is a plant commonly used in culinary and it has been indicated for their beneficial health properties, such as significant antioxidant, anti-inflammatory and hepato-protective effects. The aim of this study was to evaluate the effects of basil (Ocimum basilicum L.) by oral administration in the biochemical profile of rats. Method - Thirty male Wistar rats were randomly divided in three groups of 10 animals, and were fed ad libitum for 30 days. The first group (G1) received water ad libitum, the second and third groups (G2 and G3) received an infusion of basil at concentrations of 20 g/L and 40 g/L, respectively. Levels of glucose, total cholesterol, HDL-C, triglycerides, C-reactive protein (CRP), creatinine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were analyzed. Results - The use of O. basilicum L. decreased 37.44% in cholesterol levels and 34.55% in triglyceride levels, and increased 39.00% in HDL-c levels. Whereas the blood glycemic decreased 11.40% and 19.36% using concentrations of 20g/L and 40g/L of basil, respectively. There was no significant modification in creatinine levels, CRP and liver enzymes. Conclusion - The results show that O. basilicum L. can be helpful to prevent hyperglycemia and dyslipidemias but further studies should be done, such as the toxic analysis of basil infusion in male Wistar rats and the determination of the chemical constituents of the infusion.

Application of spectral fingerprint Identification to fumigation prearetion / 中国基层医药

Objective To establish spectrophotometrys for control the quality of fumigation preparation.Methods Two spectrophotometrys were used. 95% ethanol solutions of 5 preparations were detected by ultraviolet spectrophotometry(UV), and chloroform solutions of the preparations were detected by Fourier Transform Infrareds Spectroscopy(FIIR). Results The UV and IR spectra of the solutions were of high similarity respectively,and the absorbance peaks of spectra had the characteristic fingerprint. Conclusion Two spectrophotometrys were all simple,accurate,sensitive,which could be used for the quality control of fumigation preparation.

Avaliação do perfil lipídico, glicêmico, conteúdo de glicogênio hepático ecardíaco em ratos diabéticos suplementados com farinha de casca de maracujá (Passiflora edulis) / Assessment of the profile of lipids, glucose, liver and heart glucogen, in diabetic mice supplemented withpassion-fruit rind flour (Passiflora edulis) / Evaluación del perfil de lípidos, glucosa, contenido de glicógeno hepático y cardíaco, en ratones diabéticoscomplementados con harina de cáscara de maracuyá (Pasiflora edulis)

A ingestão de fibras alimentares está associada a redução do risco das complicações do diabetes mellitus, caracterizado pelos níveis elevados de glicose sangüínea. Com base nisto, o presente estudo propôs verificar o efeito da suplementação com farinha de casca de maracujá (Passiflora edulis) sobre os níveis plasmáticos de glicose, triglicérides, colesterol total e frações, e conteúdo de glicogênio hepático e cardíaco de ratos diabéticos. Utilizou-se 25 ratos, divididos em 5 grupos (n=5): grupo controle (GI) e grupo diabético (GII) que receberam ração comercial, dois grupos diabéticos tratados com ração suplementada com farinha de maracujá nas concentrações de 50 (GIII) e 100% (GIV) da dose correspondente à ingestão diária recomendada (IDR) de fibras, e grupo GV com ração suplementadacom 150% da IDR. Após o tratamento, coletou-se o plasma sangüíneo para análise dos parâmetros bioquímicos. No fígado e no coração, dosou-se o conteúdo de glicogênio. Em relação aos níveis lipídicos, não houve diferença significativa entre os grupos. A glicemia do GIII (137,60 ± 10,24 mg/dL)e GIV (153,60 ± 14,99 mg/dL) foi reduzida quando comparada ao GII (399,20 ± 37,21 mg/dL). O teor de glicogênio hepático e cardíaco, respectivamente, em GIII (22,91 ± 7,78 mg/g e 0,65 ± 0,09 mg/g)e GIV (28,29 ± 7,99 mg/g e 2,1 ± 0,19 mg/g) aumentou significativamente em relação ao GI (7,0 ± 4,71mg/g e 0,12 ± 0,01 mg/g), p<0,05. Conclui-se que a utilização da farinha de casca de maracujá nas concentrações de 50 e 100% da IDR foi efetiva para controle glicêmico e aumento do glicogênio hepático e cardíaco, não sendo efetiva na diminuição de lipídios plasmáticos no período de estudo.

The intake of alimentary fibers is associated to the reduction of the risk of complications with diabetes mellitus, which is characterized by high glucose levels in the blood. Based upon this,the present study aimed to check the effect of supplementation with passion fruit rind flour (Pasiflora edulis) on the glucose plasmatic levels, triglycerides, cholesterol total and fractions, and the hepatic and heart glucogen in diabetic mice. Twenty five mice were used, divided into 5 groups (n=5): control group (GI) and diabetic group (GII) which received commercial ration,two diabetic groups which received ration supplemented with passion fruit flour, 50% (GIII) and100% (GIV) concentrations of the recommended dose of daily fiber intake (RDI), and group(GV) having ration supplemented with 150% of the RDI. After the treatment, blood plasma was collected for the analysis of the biochemical parameters. The content of glucogen in the liver and the heart was quantified. In relation to the lipidic levels no significant difference was found among groups. The glucose level in GIII (137.60 + 10.24 mg/dL) and in GIV (153.60 + 14.99 mg/dL) was reduced when compared to GII (399.20 + 37.21 mg/dL). The level of hepatic and heart glucogen was significantly increased in GIII (22.91 + 7.71 mg/g and 0.65 + 0.09 mg/g) and GVI (28.29 + 7.99 mg/g) when compared to GI (7.0 + 4.71 mg/g and 0.12 + 0.01 mg/g), p<0.05. It was concluded that the use of flour from passion fruit rind in the concentrations of 50% and 100% was effective on the control of blood glucose and the increase of hepatic and heart glucogen, not being effective on the reduction of plasmatic lipids during the period of study.

La ingestión de fibras alimentares está asociada a la reducción del riesgo de complicaciones de la diabetes mellitus, caracterizada por elevados niveles de glucosa en la sangre. Con base en esto, el presente estudio se propuso verificar el efecto de la complementación con harina de cáscara de maracuyá (Pasiflora edulis) sobre los niveles plasmáticos de glucosa, triglicéridos, colesterol total y fracciones, y contenido de glicógeno hepático y cardíaco en ratones diabéticos. Se utilizaron 25 ratones, divididos en 5 grupos (n=5): grupo de controle (GI) y grupo diabético (GII) que recibieron ración comercial, dos grupos diabéticos tratados con ración complementada con harina de maracuyá, en las concentraciones de 50% (GIII) y 100% (IV) de la dosis correspondiente a la ingestión diaria recomendada (IDR) de fibras, y grupo (GV) con ración complementada con 150% de la IDR. Tras el tratamiento se colectó el plasma de la sangre para el análisis de los parámetros bioquímicos. En el hígado y en el corazón se ha dosificado el contenido de glicógeno. Con relación a los niveles lípidos no hubo diferencia significativa entre los grupos. La glicemia del GIII (137,60 + 10,24 mg/dL) y GIV (153,60 + 14,99 mg/dL)se ha reducido cuando comparada con GII (399,20 + 37,21 mg/dL). El contenido de glicógenohepático y cardíaco, respectivamente, en GIII (22,91 + 7,78 mg/g y 0,65 + 0,09 mg/g) y GIV(28,29 + 7,99 mg/g y 2,1 + 0,19 mg/g) ha aumentado significativamente con relación al GI (7,0+ 4,71 mg/g), p<0,05. Se ha concluido que la utilización de la harina de cáscara de maracuyá,en las concentraciones de 50% y 100% de la IDR, fue efectiva para el control de la glucosa y del aumento del glicógeno hepático y cardíaco, no resultando efectivo en la reducción de lípidos plasmáticos durante el periodo del estudio.

Effect of compound preparation of Astragalus mongholicus on subsets of lymphocytes in patients with myasthenia gravis / 国际检验医学杂志

Objective To investigate the effect of compound preparation of Astragalus mongho-licus on subsets of lymphocytes in patients with myasthenia gravis (MG), and to explore its regulatory mechanism. Methods 60 MG patients were randomly divided into test group (n=30, given compoundpreparation of Astragalus mongholicus for 12 wks) and control group (n=30, given prednisone tablets for 12 wks). Flow cytometry was applied to examining the distribution change of the subsets of lym-phocytes in patients before and after treatment. Results After treatment, the percentages of CD4+ T cells and the ratio of CD4+/CD8+ in both groups decreased compared with those before treatment.The ratio of CD4+/CD8+ in test group was significantly lower than that in control group (P<0.05).The percentage of CD8+ T cells in test group after treatment increased significantly (P<0.05) com-pared with that before treatment, but had no significant difference compared with that in control group(P>0. 05). Conclusion The result from the current study suggests that one of the mechanisms of the compound preparation of Astragalus monghoticus regulating immune response may be achieved through its modulating effect on the distribution of subsets of lymphoeytes and humoral immune func-tion.

Study on anti-tumor mechanism of Chinese medicine Fuzhengyiliufufang by molecular docking method / 中国临床药理学与治疗学

AIM:To investigate the anti-tumor effects of Chinese medicine Fuzhengyiliufufang(FZYLFF) and its mechanism.METHODS:Molecular docking was apllied to simulate the interactions between Chinese medicine small molecules and TNF-?,IL-2 receptors respectively,with the aid of ligand-fit module in the software package Cerius2 4.10 of Accelrys company,to predict the effects of FZYLFF on anti-tumor.RESULTS:According to the dockscore of original ligand and the receptor as threshold value,thirty-seven molecules were predicted to have good interactions with TNF-? and ten molecules with IL-2.CONCLUSION:FZYLFF is a promising Chinese medicine for tumor therapy.Its mechanism is possibly attributed to indirect inhibition by interfering inflammatory cell factors and enhancing immunoregulation.

In vitro effects of compound Chinese herbal medicine Zilongjin on prostate cancer cell lines / 中华泌尿外科杂志

Objective To investigate the in vitro effects of compound Chinese herbal medicine Zilongjin on prostate cancer cell lines. Methods MTT assay,flow cytometry, colony on soft agar,and fluorescence microscopy were used to observe the effects of Zilongjin on anti proliferation,inhibition of clonal growth,cell cycle arrest and apoptosis induction.RT PCR method was used to study the regulative effect of Zilongjin on prostate marker genes PSA,AR,apoptosis related genes Bcl 2,Bax and tumor suppressing gene p16. Results Zilongjin caused notable anti proliferative effect,and induced G 0/G 1 phase arrest dose dependently on LNCaP,DU 145 and PC 3 cell lines.Inhibition of cell growth by 50% (IC 50 ) was observed with Zilongjin at concentrations of 0.79,0.42 and 0.52 mg/ml,respectively.Apoptosis induction was seen in LNCaP and DU 145 cell lines.Zilongjin also caused inhibition of clonal growth of DU 145 cell line.In addition,Zilongjin down regulated the expression of PSA,AR and Bcl 2 genes in LNCaP cell line;and down regulated Bcl 2,up regulated Bax and p16 genes expression in DU 145 cell line. Conclusions It is demonstrated that Zilongjin has antitumor action through anti proliferation,inhibition of clonal growth,G 0/G 1 phase arrest,apoptosis induction and regulating the expression of PSA,AR,Bcl 2,Bax and p16 genes.